HPLC metabolomics as a quick tool to identify antibacterial compounds from Solanum torvum Sw.

Nancy Dewi Yuliana, Nuri Andarwulan, Dias Indrasti

SEAFAST Center, IPB, Bogor and Dept. of Food Science & Technology, Fateta, IPB, Bogor

ABSTRACT

The natural occurance of plant extract as a complex mixture of compounds with a broad range of polarity, melting/boiling point, and concentration, complicates the identification of the bioactives from these distnguish source. Metabolomics as a new emerging approach is viewed as a potent tool to solve the abovementioned problems. The research aims to identify antibacterial compounds from Solanum tuberosum by using HPLC metabolomics and orthogonal projection to the least square (OPLS) analysis. Two blocks of data resulted from this research, i.e. antibacterial activity and HPLC chromatograms were correlated by OPLS. Several peaks were found to be positively correlated with antibacterial activity, those are 16.20 to 16.75 minutes with VIP 0.35 – 0.68, 30.04 to 30.33 minutes with VIP 0.60 – 0.86 , 31.40 – 31.70 minutes with VIP 0.60 – 0.90. These peaks were detected at UV wavelenght of 220, 270 330, and 360 nm. The peaks are found to be abundant in the first five fractions which are catagorized as active group. The peaks will be further analyzed by LC-MS to elucidate the possible active compounds.